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All content of this sequence listing is incorporated herein by reference for all purposes. El listado de secuencias se identifica en el archivo. In alternative embodiments, the invention provides phospholipase C enzymes specific to Phosphatidylinositol PI-PLCnucleic acids encoding these antibodies intoxiacion specifically bind to them, and methods for obtaining them and use them.
Industrial methods and products comprising the use of these phospholipases are provided. In certain kuriatico, are methods of hydration followed NHPs enzymatic treatment and removal of various phospholipids and lecithins are provided. The methods provided herein can be practiced on either oil or water degummed gross In certain embodiment, methods are provided here for phospholipids from edible oil.
The removal of phospholipids generates almost all losses associated with refined vegetable oils. Grupos funcionales Functional groups. La Tabla 1 contiene las by water, while q ue the acids, acid mugiatico calcium Camagnesium Mgand iron Fe “and ethanolamine salts Ca, Mg, and Fe have the much lower affinities water. Table 1 contains the. Salts of calcium, magnesium, iron and phospholipids are formed by an enzyme present in oilseeds, phospholipase D PLD.
The enzyme pennanece latent within the mature seed intoxicacioj the protective seed coat has been damaged during storage or “preparations” seed prior to deoiling. Additionally, because the cleavage occurs in the presence of an abundance of aido metal Ca, Mg, and FeNHPs are formed. Niveles tipicos y distribuciones de los fosfolipidos para las semillas oleaginosas comunes Table 2: Typical levels and distributions of phospholipids for common oilseeds.
Table 3 below provides typical amounts of phospholipids and distributions for soybean gums. Typical amounts of Phospholipids and distributions for Gums Soja. The conversion of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and phosphatidic acid in any of its forms or phospho smooth greatly changes the economics degumming in a modern industrial refining operation. The conversion of all phospholipids in their lyso forms eliminating the loss of neutral oil an increase in oil yield up to 1.
Phospholipids may be partially or totally removed from vegetable intoxkcacion through several different known means. The most commonly used in industry procedures are water degumming, acid degumming with, caustic refining and enzymatic degumming.
Procedimientos ejemplares se describen en las Patentes US nos 4. S f, f, f and f Existing methods are not sufficient to eliminate or to react the non-hydratable phospholipids present in the oil because the NHPs are not available to be hydrated or reacted to enable removal. Phospholipases are enzymes which hydrolyze ester bonds of phospholipids. Correspondiendo a su importancia en el metabolismo de los fosfolipidos, estas enzimas se esparcen entre las procariotas y eucariotas.
Corresponding to their importance in the metabolism of phospholipid, these enzymes spread between prokaryotes and eukaryotes. Phospholipases affect metabolism, construction and reorganization of biological membranes and are involved in signal cascades. Phospholipase C enzymes specific to Phosphatidylinositol PI-PLC are a family of eukaryotic intracellular enzymes that play an important role in signal transduction processes.
Las familias de las enzimas de fosfolipasa C PLC se han identificado en bacterias y en tripanosomas eucariotas. Families of enzymes phospholipase C PLC have been identified in bacteria and eukaryotes trypanosomes.
Las enzimas PLC pertenecen a la familia de las hidrolasas y las fosfodiesterasas. The PLC enzymes belong to the family of hydrolases and phosphodiesterases. The PLC is involved in the metabolism of phosphatidylinositol 4,5-bisphosphate PIP2 and signaling pathways of lipid in a calcium dependent manner.
Limpiador de Pocetas MAS: el comercial récord
PLC isoforms can differ in their mode of activation, expression levels, catalytic regulation, cellular localization, avidity of membrane binding and tissue distribution. All are capable of catalyzing the hydrolysis of PIP2 in two important second messenger molecules, which come to alter cell responses such as proliferation, differentiation, apoptosis, cytoskeletal remodeling, vesicular trafficking, ion channel conductance, endocrine function and neurotransmission.
Las Pl Cs se describen en, por muraitico, Carmen, G. Chem,Inoxicacion, D. Cs Pl are described in, for example, Carmen, G. Chem,Wagoner, D. Phospholipase A2 enzymes PLA2 removes the fatty acid from position 2 to produce free fatty acid and 1-acyllysophospholipid.
Intracellular PLA2 is found in almost all mammalian cells. Enzymes phospholipase C PLC remove the phosphate radical to produce 1,2-diacylglycerol and a phosphate ester. In one aspect, polypeptides are provided herein, for example, enzymes, having a intoxicacoon activity, e. In another aspect, the polypeptides as provided herein are used to synthesize enantiomerically pure chiral products.
The polypeptides provided herein can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In addition, the polypeptides provided herein can be used in food processing, brewing, acidoo additives, alcohol production, peptide synthesis, enantioselectivity, skin preparation animal in the leather industry, management waste and degradation of intoxkcacion, recovery of silver in the photographic industry, medical treatment, degumming of silk, degradation of biofilms, biomass conversion to ethanol, biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, reagents biotechnology waste in increasing the yield of starch from the corn wet milling, and as pharmaceuticals such as digestive aids and antiphlogistics anti-inflammatory agents.
Any oil, for example vegetable oil, for example canola oil, soybean oil, or animal oil or fat, for example tallow, may be treated with a composition, or by a method as provided herein. Animal intoxicacoin which has been treated with a composition or by a method as provided herein. Vegetable oils modified to be oils with low phospholipid content can be used in any food, foodstuff or baking or cooking, for example, sauces, marinades, condiments, spray intoxucacion, margarines, oils baking, mayonnaise, oil product cooking, salad oils, dressings applied with spoon and pourable, and the like.
Sintomas de intoxicacion por cloro y acido muriatico wikipedia
In one embodiment, here oils such as vegetable oils, for example, canola oil or soybean oil, and food or baked or cooked products, including sauces, marinades, condiments, spray oils, margarines, mayonnaise, oils are provided baking oils cooking, frying oils, salad oils, dressings applied spoon and pr, and the like, where the oil or food, baked or cooked has been modified using an enzyme as provided herein. In one aspect, they provided herein are polypeptides, eg, enzymes and catalytic antibodies, having a phospholipase activity, e.
In one muriatifo, the recombinant isolated nucleic acid, synthetic or encodes a polypeptide or peptide having a phospholipase activity, eg a phospholipase and, eg a phospholipase and specific to Phosphatidylinositol PI-PLCwhich is thermostable.
In one embodiment, a nucleic acid probe, for example, a probe to identify a nucleic acid encoding a polypeptide having a phospholipase activity, e. In one embodiment, a pair of sequences of amplification primers to amplify a nuc eico acid!
Encoding a polypeptide having a phospholipase activity, for example phospholipase and, for example phospholipase C specific to Phosphatidylinositol PI-PLCcomprising a primer pair comprising or consisting of a pair of primers capable of amplifying a nuc acid! In one embodiment, methods for amplifying a nucleic acid encoding a polypeptide having a phospholipase activity, for example phospholipase and, for example phospholipase and specific to Phosphatidylinositol PI-PLCcomprising amplification of a template nucleic acid with a sequence pair of amplification primers capable of amplifying a sequence of nuc acids!
In one embodiment, vectors, expression cassettes, expression vectors, plasmids, or cloning vehicles comprising a nucleic acid as provided herein or a subsequence thereof. In one embodiment, the expression cassettes comprising a nucleic acid as provided herein or a subsequence thereof.
In one aspect, the expression cassette can comprise the nucleic acid is linked operably to a promoter. The promoter may be a viral, bacterial, mammalian or plant promoter.
In one aspect, the plant promoter may be a promoter from potato, rice, corn, wheat, barley or snuff. El promotor puede ser un promotor constitutivo. The promoter may be a intxicacion promoter. El promotor constitutivo puede comprender CaMV35S. The constitutive promoter can comprise CaMV35S.
En otro aspecto, el promotor puede ser un promotor inducible. In another aspect, the promoter may be an inducible promoter.
INTOXICACIÓN POR SUSTANCIAS CORROSIVAS by JESSICA DEFAZ CHICAIZA on Prezi Next
In one aspect, the promoter may be a tissue specific promoter or a promoter to environmentally regulated or a regulated promoter under development. Thus, the promoter can be, for example, a promoter specific intoxicqcion the seed-specific promoter to the sheet, a promoter specific to the root, the stem specific promoter or a promoter induced abscission.
In one embodiment, a host cell or a transformed cell comprising an acid nuc! Eico as provided herein. In one aspect, the host cell or a transformed cell can be a bacterial cell, a mammalian cell, a fungal cell, a yeast cell, an insect cell or a plant cell.
In one aspect, the plant cell may be a cell of potato, wheat, muriativo, maize, barley or snuff. The transformed cell may be any of the kuriatico cells familiar to those skilled in the art, including prokaryotic cells, eukaryotic, such as bacterial cells, fungal cells, yeast cells, mammalian cells, insect cells, or plant cells. Muriaticl fungal cells include any species of Aspergillus yeast cells include any species Exemplary Pichia, Saccharomyces, Schizosaccharomyces, Schwanniomyces or including Pichia pastoris, Saccharomyces cerevisiae, or Schizosaccharomyces pombe.
In another embodiment, transgenic non-human animals comprising a nucleic acid as provided herein or a vector, expression cassette, expression vector, plasmid or cloning vehicle as provided herein. The transgenic nonhuman animal may be a mouse, a rat, a goat, a rabbit, a sheep, a pig or a cow.
In one embodiment, a seed or transgenic plant comprising a nucleic acid as provided herein or a vector, expression cassette, expression vector, plasmid or cloning vehicle as provided herein.
In one embodiment, the seed is a corn seed, a wheat seed, an oil seed, rapeseed seed, soybean seed, palm, a sunflower seed, a sesame seed, a seed of rice a barley seed, a seed, peanut, cotton seed, palm seed, a seed, peanut, sesame seed, sunflower seed or plant seed snuff. In one aspect, there is provided an antisense RNA inhibitor or comprising or consisting of a nucleic acid as provided herein oligonucleotide. In another aspect, there is provided a method for inhibiting the translation of a message phospholipase transcribed, mRNA in a cell, comprising administering to the cell or expressing in the cell an antisense oligonucleotide or RNA inhibitor comprising or consisting a nucleic acid sequence provided herein.
Limpiador de Pocetas MAS: el comercial récord – Cuando era Chamo – Recuerdos de Venezuela
A comprising an amino acid sequence: In one aspect, the isolated, synthetic or recombinant polypeptide can comprise the polypeptide as provided herein lacks a signal sequence peptidesfor example, lacking its signal sequence homologous, and in one aspect, comprises a signal sequence peptides heterologous.
In one aspect, the isolated, synthetic or recombinant polypeptide can comprise the polypeptide as provided herein comprising a heterologous signal sequence, such as a signal sequence phospholipase or phospholipase eg, not phospholipase, not phospholipase C or phospholipase C specific to phosphatidylinositol PI-PLC heterologous.
In one aspect, murriatico chimeric proteins comprising a first domain comprising a signal sequence as provided herein and at least a second domain.
The protein can be a fusion protein. El segundo dominio puede comprender una enzima. The second domain can comprise an enzyme. The enzyme may be a phospholipase, eg a phospholipase C, for example, a specific phospholipase C phosphatidylinositol PI-PLC as provided herein, or another pog. In one aspect, the phospholipase activity, e. In another aspect, the phospholipase activity, e. In one aspect, the phospholipase activity comprises a specific activity at C in the range from about to about units per milligram protein.
In one embodiment, the isolated, synthetic or recombinant polypeptides as provided herein comprise at least one glycosylation site. PAMBE or plants, such as oil-producing plants such soybean, canola, rice, sunflower, or genetically modified variants GMO of these plants.
In one aspect, the polypeptide can retain a phospholipase for example phospholipase C, par example specific phospholipase C phosphatidylinositol PI-PLC under conditions comprising about pH 6. In another aspect, the polypeptide can retain a phospholipase activity, e. In one embodiment, the protein preparations comprising a polypeptide as provided herein, wherein the protein preparation comprises a liquid, a solid or a gel. In one aspect, the heterodimers as provided herein comprise a polypeptide and a second domain.